مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Envaluation of IL-22 gene polymorphisms in rs867810424 and rs 1390124543 positions in infertile women
Envaluation of IL-22 gene polymorphisms in rs867810424 and rs 1390124543 positions in infertile women
sirous naeimi,1,*Farideh Orooji,2
1. Faculty Member of Kazeroun Azad University 2. she has been working as a therapeutic expert at Shiraz University of Medical Sciences.
Introduction: fertility is one of the great goals of creation for the survival of the generation, which is of particular importance. infertility means having a couple unprotected for one year and not getting a pregnancy. lack of fertility is very important in terms of social and family issues. The imbalance of the immune cells in the uterus and the dysfunction of the cytokines may lead to pregnancy loss.The purpose of this study was to investigate the effect of polymorphisms of the immune system genes on infertility in women. rs867810424 and rs 1390124543 in IL-22 gene with the risk of infertility in women , can be used as screening , prevention and timely treatment in infertile women.
Methods: General information was collected by completing the questionnaire forms . DNA extraction of the samples was performed by DNA salting out method. Genotypes were determined by PCR- ARMS method and the results were analyzed by logistic regression test.
Results: According to the results, It is possible that rs 867810424 and rs1390124543 polymorphisms in the IL-22 gene are associated with infertility and these findings can be used as a finding. predictive factor in use.
Conclusion: In this case - control study, 200 healthy individuals and 200 infertile patients were evaluated according to clinical and laboratory findings in the age range of 20-45(+_ 5) who referred to infertility treatment centers. General information was collected by completing forms. the result of this study showed that at the genetic level , both rs867810424 and ra1390124543 polymorphisms in IL-22 gene were significantly associated whit female infertility.there was no significant relationship between the rs867810424 and rs 1390124543 alleles of the IL-22 gene whit female infertility.Influence of alleles on infertility in dominant, recessive and heterozygous states in the rs867810424 and rs1390124543 polymorphisms of the IL-22 gene showed that each of the genotypic states was associated withe infertility in women compared to the other states. and the relationship is meaningful.