Introduction: Cardio vascular diseases are the most important of mortality causes in the world. Genetic factors are non-modifiable risk factor in coronary atherosclerosis. NOS3 gene is one of important genes which its polymorphisms and association to coronary arterial diseases has proved. Nitric oxide is generating by NOS enzymes family. NOS enzyme is present in endothelial cells produces by NOS3 gene. Our investigation is on T786C polymorphism (rs2070744) which is located on promoter region of NOS3 gene to illustrate its association with coronary atherosclerosis in Iranian patients.
Methods: Overall 160 people participate in our study, including 80 persons diagnosed for coronary atherosclerosis who admitted at Afshar Hospital (Heart center), Yazd, Yazd province as patient group and 80 control healthy individuals participate as control group.
Blood samples were collected and DNA of blood samples extracted using salting out procedure. After Gel Electrophoresis and conducting Gel Documentation, detection of T786C polymorphism has done by MULTIPLEX ARMS-PCR. Results analyzed by SPSS software as statistical analyzing tool.
Results: Analyzing resulted data shows that T786C polymorphism (rs2070744) of NOS3 gene is associated to coronary atherosclerosis occurrence.
Statistical analysis indicates significant differences in TT and TC genotype frequency in comparison of CC genotype in patient and control groups. (p=0.014, 95%CI=0.1039). Also, there is significant differences between T and C allele frequency in patient and control groups. (p=0.0001, 95%CI=0.252).
In patient group TT, TC and CC genotype frequencies are 56.25%,33.75% and 10% respectively. whereas in control group TT, TC, CC genotype frequencies are 80%,18.75% and 1.25% respectively.
Conclusion: Previous studies declared association between NOS3 gene variation and different disease appearances such as immune system disfunctions. In conclusion present study reveal that T786C polymorphism (rs2070744) in NOS3 gene is positively associated to coronary atherosclerosis. Results of Gel documentation showed that frequency of TT is 45 (56.25%) and 64(80%) for patient and control participants respectively. 27 (33.25%) patients and 15 (18.75%) persons of control group had TC genotype. Also 8 (10%) persons of patient group and 1(1.5%) person of control group had CC genotype .Statistical analysis results, using SPSS software, showed a significant difference in frequency of TT and TC genotypes in compare of CC genotype in patient and control groups (p = 0.014, 95%CI=0.1039). Also, allele frequency differences for T and C alleles are significant in patient and control groups. (P = 0.0001, 95%CI=0.252).