• Evaluation of lncRNA FER1L4 expression in the colorectal cancer
  • Marjan Ostovar Pour ,1 Mohammad Khalaj Kondori,2,* Tayyebeh Ghasemi ,3
    1. Dept. of Genetics, Animal Biology Group, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
    2. Dept. of Genetics, Animal Biology Group, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
    3. Dept. of Genetics, Animal Biology Group, Faculty of Natural Science, University of Tabriz, Tabriz, Iran


  • Introduction: Colorectal cancer (CRC) as a gastrointestinal malignancy is the fourth most common cancer and also the third leading cause of cancer-related deaths in the world. Distant metastasis and recurrence of disease are the cause of most CRC-related deaths. So, markers are needed to identify this cancer in early stages. Some long noncoding RNAs (lncRNAs) demonstrated to be involved in gene expression regulation and tumorigenesis. In this study, we evaluated the expression level of Fer-1-like protein 4 (FER1L4) in colorectal cancer tissues compared with matched adjacent normal tissues.
  • Methods: Total RNA was extracted from forty-six pairs of colorectal tumor and their marginal tissue samples using RNX-plus reagent and cDNA was synthesized by PrimeScript RT reagent kit (TaKaRa). Expression of FER1L4 was evaluated by qRT-PCR. U6 snRNA was used as internal control gene.
  • Results: FER1L4 LncRNA expression was significantly decreased in tumor tissue compared with non-cancerous tissues (fold change: 0.27, P-value: 0.002).
  • Conclusion: Results of this study highlighted that FER1L4 expression might be a potent biomarker for CRC.
  • Keywords: Colorectal cancer, FER1L4, tumor suppressor, down-regulated