مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Synergic Effect of Doxorubicin and Lignan, a Natural Anticancer Agent, on Prostate Cancer
Synergic Effect of Doxorubicin and Lignan, a Natural Anticancer Agent, on Prostate Cancer
Maryam Hakimi,1Siamak Ebrahimi,2Shirin Farivar,3,*
1. Life Sciences and Biotechnology Faculty of Shahid Beheshti University 2. Life Sciences and Biotechnology Faculty of Shahid Beheshti University 3. Life Sciences and Biotechnology Faculty of Shahid Beheshti University
Introduction: Prostate cancer is one of the most malignant and common diseases in men, a gland in men reproductive system. It is treatable if diagnosed at early stage. Doxorubicin is used to treatment although, it is not in first-line therapy of prostate cancer. Moreover, it has showed many side-effects as like as other synthetic anticancer agent. Because of this, many laboratories are using drug synergy to decrease the usage dose of doxorubicin in prostate cancer patients. Doxorubicin can induce apoptosis by affecting the gene expression of different proteins. Phytochemicals such as plant polyphenols are considered to have anticancer effect. Polyphenols have the ability to modulate multiple targets of carcinogenesis linking models of cancer characteristics by having effect on cellular and molecular targets. Lignans are an important group of polyphenols, exists in high levels in some foods such as flaxseed. Here in this article tried to illustrate the synergic effect of doxorubicin and flaxseed on two major pathways which are involved in prostate cancer. There are different researches which are focused on some target molecules separately studied on doxorubicin and lignan but there is no study to compare and highlight on both. Some of these molecules were Bcl-2, p-p53, cyt-C and caspase-3. These molecules have an important role in cellular signaling pathways such as: PI3-Akt and p53 pathways.
Methods: According to the literature there are some molecules which are affected with both doxorubicin and lignan, and then the two major pathways involving at prostate cancer studied by using Kegg. By using Kegg pathway the related molecules involving at both pathways were investigated. Moreover, the role of these specific molecules on prostate cancer and how they are trigger by doxorubicin and lignan and also their synergic effects were studied.
Results: Both doxorubicin and lignan derived from flaxseed, down-regulate the expression of Bcl-2 gene. Doxorubicin and lignan can also up-regulate the expression of p53 gene, resulted in inducing of apoptosis in prostate cancer cells. Besides that, they can induce p53 signaling pathway apoptosis by affecting on p53, cyt-C, and caspase 3. They also have effected on PI3K signaling pathway via using caspase-9 protein and Bcl-2.
Conclusion: Our study showed that doxorubicin and lignan, have synergic affect and can be used with each other to reduce the usage dose of doxorubicin. By using the lignan and doxorubicin the side effects of doxorubicin can be reduced which it causes in better managing and fastest recovery for patients.