مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
LPS induces microRNAs -9, -192 and -205 overexpression in colorectal cell lines
LPS induces microRNAs -9, -192 and -205 overexpression in colorectal cell lines
Ebrahim Faghihloo,1,*Shaian Tavakolian,2
1. Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2. Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Introduction: Nowadays, Cancer is one of the serious health problems in the world, and colorectal cancer is the third cause of death in the American society. It has reported, some risk factors, including family history of colorectal cancer, age and infectious diseases can deteriorate the progression of colorectal cancer. One of the common Specifications in all negative-gram bacteria is their bacterial cell wall, known lipopolysaccharide [LPS]. Moreover, probably LPS can have effects on some microRNAs, and cancer progression, subsequently. We have studied the effects of LPS on microRNA-9, -192 and microRNA-205 expressions on colorectal cell lines [SW480, HCT116]
Methods: SW480 and HCT116 cell lines have been treated with LPS to analyze microRNA-9,192 and 205 expressions by quantitative real-time PCR in 48 hours at 10ug/L of LPS
Results: Quantitative real-time PCR has illustrated, microRNA-9,-192 and -205 are up-regulated after treating LPS. There is an upward trend in microRNA-9 level six and eight times in SW480 and HCT116 cell lines, respectively. Furthermore, increase in the expression of microRNA-192 has observed six times in HCT116 and four times in SW480; moreover it has been shown a same increasing level [almost four times in both cell lines] in microRNA-205
Conclusion: Our results show, treating LPS leads to an increase in microRNA-9, -192 and -205, related to cancer in colorectal cell lines [SW480 and HCT116]. Therefore it is possible, disrupting the balance of bacterial flora playing a role in colorectal cancer progression, and increase the chances of getting colorectal cancer that further investigation is required.