مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Evaluation of macrophage functions after adjacency with LPS-primed mesenchymal stem cells treated with Pistacia atlantica gum extract
Evaluation of macrophage functions after adjacency with LPS-primed mesenchymal stem cells treated with Pistacia atlantica gum extract
Hadi Esmaeili Gouvarchin Galeh,1,*Mahdieh farzanehpour,2Nader Nezamdoost Shadbad,3Masoumeh Bolandian,4
1. Applied Virology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran. 2. Applied Virology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran. 3. Applied Virology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran. 4. Applied Virology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran.
Introduction: Precursor monocytes are differentiated into macrophages and their function through the regulation of tissue microenvironment. Macrophages undergo differentiation into discrete functional populations to respond the existence of microbial products, stimulated lymphocytes, or damaged cells in the microenvironment. The current research aimed to examine the impact of Pistacia atlantica gum extract on the interplay of lipopolysaccharide activated MSCs with macrophage functions
Methods: Methods: In this experiment, mesenchymal stem cells were isolated from bone marrow of BALB/c mice and then induced with 10 ng/mL of LPS for 1 h. LPS cells were harvested after discarding and washing out the supernatant. Thereafter, the cells were subjected to incubation at various concentrations of P. atlantica gum extract (100, 200, and 400 µg/ml) within 24 h. Subsequently, the co-culture of mesenchymal stem cells was performed with macrophage followed by incubation during extra 4 h, and final evaluation of the macrophage function. SPSS software was used to analyze the data at a statistical significance of P < 0.05.
Results: Results: According to the findings, the use of P. atlantica gum extract to treat pro-inflammatory mesenchymal stem cells led to boosted reduction rate of Neutral Red-uptake and respiratory burst of activated macrophage depending on treatment doses.
Conclusion: Conclusion: Application of P. atlantica gum extract to treat mesenchymal stem cells in inflammatory condition can strengthen the function of mesenchymal stem cells in reducing phagocytosis and the respiratory burst of macrophages cells.