• Evaluation Epstein-Barr virus in children under 5 years suspected mononucleous city of Sanandaj
  • Pezhman Sharifi,1,* Woria Babahajian,2
    1. Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
    2. Blood Transfusion Research Center, High institute for Research and Education in Transfusion Medicine, Tehran, Iran


  • Introduction: Epstein-Barr virus of the herpes virus family and is the subfamily Gamma herpes Virinae Infection with the virus is common in the whole world. In developing countries, more than 90% of children, up to 6 months old, become infected. The virus causes infectious mononucleosis, which is associated with the involvement of b-lymphocytes and symptoms such as headache, restlessness, fatigue, and sore throat.
  • Methods: Out of 18 children with suspected cases of infectious mononucleosis, blood samples were taken from the medical examination laboratory of Hosseini Nasab in Sanandaj And their serum was separated after 15 minutes of centrifugation. Then they tested IgM anti–VCA by the closed system Alegria company ELISA was performed. To confirm the positive cases, Monotest (rapid method) and the method of observing the patient's blood smears, under the microscope, are used to observe the presence and amount of large lymphocytes (activated T cells) known as atypical cells. These cells are found in viral infections such as mononucleosis, mumps, cytomegalovirus, and hepatitis, also can be seen in drug reactions and pertussis, and brucellosis.
  • Results: In 10 cases (16.67%) of boys and 8 patients (13.34%) of the girls, who had mononucleosis symptoms IgM test was positive (30% total n = 18). Rapid serologic testing (Monotest) was positive in all of them (100%). In peripheral blood smear, there were atypical lymphocytes with an average of about 3.2 atypical cells per patient.
  • Conclusion: As can be seen, all of these tests are good confirmation tests and Performing a Monotest and observing atypical cells can help in cases of positive antibodies IgM EBV and suspicion of infectious mononucleosis. But it must be said that for the final diagnosis of infectious mononucleosis, especially in acute cases, other tests, such as WoW and Nenenen, are necessary with new methods and techniques and closed systems.
  • Keywords: Epstein-Barr virus, children, suspected mononucleous, Sanandaj