• Investigate of Ptomoter methylation of PTGS2 gene in blood samples belong women with PCOS using the methylation specific PCR method
  • Saeideh Amini,1,* Seyed Hamid Jamaldini Ezabadi,2 Gholamreza Bahrami Monjami,3
    1. Department of genetic, Faculty of Advanced Sciences & Technology,Tehran Medical Sciences Branch, Islamic Azad University,Tehran, Iran
    2. Department of genetic, Faculty of Advanced Sciences & Technology,Tehran Medical Sciences Branch, Islamic Azad University,Tehran, Iran


  • Introduction: Polycystic ovary syndrome is the most common cause of ovulatory disorder. oligoanovulation and changes in oocyte's competence are related with increased risk for infertility in this disease. One of the genes involved in this process and expressed by cumulus cells, is PTGS2. Change in expression of this gene can lead to ovulation disorder and decrease oocyte competence and change in methylation pattrern is one of the Epigenetic mechanisms that alter gene expression. so, We examined the methylation of one of the CPGs at the site of NF-IL6 in the PTGS2 gene promoter with PCOS phenotype in blood sample of individuals to determine if it can be used as a marker for diagnosis and prediction of PCOS.
  • Methods: The statistical population included the blood samples of 50 woman with PCOS and 50 woman without PCOS.After extracted the genomic DNA of the blood sample, DNA was treated with bisulfite. Then, using the Methylation Specific PCR technique, samples were amplified. In next step samples were run on polyachrilamid gel and painted.
  • Results: all of the patients and normal group's person are hypermetylation in this CPG.
  • Conclusion: According to studies done PTGS2 gene plays a role in oocyte development and ovulation, but based on the results, there is not present significant difference between the patient group and normal control in terms of the pattern Methylation in this region and this CPG in the promoter region of the PTGS2 gene is probably not associated with PCOS . Therefore, it is suggested that in future studies, choose more samples for this research and to save time and cost, researchers do not instruct this region of PTGS2 gene for their study and study other CpG sites in the PTGS2 gene to can accurately reject or accept the role of DNA methylation of PTGS2 gene in the development of PCOS and find a right and quick marker for PCOS.
  • Keywords: Polycystic Ovarian Syndrom (PCOS), infertility, anovolution, PTGS2,Methylation Specific PCR(MSP)