مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
The Evaluation of Cytotoxicity and Antioxidant Compounds of an Orange Fruits Extract of Physalis alkekengi on Human Lung Cancer A549 Cell Line
The Evaluation of Cytotoxicity and Antioxidant Compounds of an Orange Fruits Extract of Physalis alkekengi on Human Lung Cancer A549 Cell Line
Mahdieh Houshani,1,*Babak Babakhani,2Mohammad Masoomifar,3
2. Department of Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran 3. Department of Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
Introduction: The Reactive Oxygen Species (ROS) damage to cells led to the development and the progression of many disease such as cancer, gout, aging, diabetes, and cardiovascular disease. Lung cancer is the most common cancers and chemotherapy cancer treatment is one of the methods, but because of the lack of selective cytotoxicity, it leads to the occurrence of intolerable side of the screw. Thus recently, natural products extracted from medicinal plants can play an important role in cancer treatment. Based on medicinal effect of the Physalis alkekengi, it was aimed in this study to determine the content of some antioxidant compounds of its fruits and the inhibitory effects of their crude extract on lung cancer A549 cell line, too
Methods: Therefore, an orange fruit of Physalis alkekengi was harvested from the region of Abbas Abad city and A549 cells line was cultivated and proliferated. Then, the cells exposed to different concentrations of the fruits extract of Physalis alkekengi (31.25 to 2000 μg/ml) were incubed for 24, 48, and 72 hours. After the incubation period, the colorimetric MTT method was used to determine cytotoxicity. Also, total phenol, flavonoid, anthocyanin, and carotenoid contents were determined
Results: The results showed that the value of total phenol, flavonoids, and anthocyanins were 8.94±1.25, 0.231±0.251 and, 10.85±1.01 mg g-1 DW, respectably and the value for carotenoid contents was 10.05±1.21 mg g-1 FW. The results have also shown that the extract of fruits brought the highest inhibition of cancer cell growth at the concentration level of 2000 μg/ml that was 91.81%.
Conclusion: These results suggest that the orang fruit extract of Physalis alkekengi have the most antioxidant and cytotoxicity against A549 cell line. It seems, the orang fruit extracts of Physalis alkekengi are recommended as the most appreciate tissues for pharmacological studies and cancer treatments.