مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Effect of Decitabine on the Expression of DNMTs in HL-60, NALM-6 and Normal Blood Cells
Effect of Decitabine on the Expression of DNMTs in HL-60, NALM-6 and Normal Blood Cells
Sina Dalvand,1,*Amin Namdari,2
1. International Campus of Shahid Sadoughi University of Medical Sciences, Yazd, Iran 2. Department of Clinical Biochemistry, Fasa University of Medical Sciences, Fasa, Fars, Iran
Introduction: The 5-aza-2'-deoxycytidine (Decitabine (DAC)) is a deoxycytidine analog currently being used as an effective drug against myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). Although various studies have pointed out to the epigenetic effects of this drug, but researchers have not yet clearly defined or specify the epigenetic mechanisms by DAC in different cell lines. In this study, a possible epigenetic effect of DAC on leukemias' cell lines NALM-6 and HL-60 was assessed and then compared to the normal blood cells PBMCs.
Methods: At the logarithmic phase, cultured cells HL-60 and NALM-6 were treated with a selected dose of DAC defined from literature. The gene expression of epigenetic enzymes DNMTs was assayed with the Real Time PCR technique, before and after the treatment. Additionally, MTT assay was also done to assess the viability of treated cells.
Results: The in vitro findings showed that the gene expressions of DNMT1 and DNMT3B were significantly higher in leukemia cell lines than those in PBMCs. The gene expression of these enzymes decreased significantly, after treatment with DAC.
Conclusion: One of the mechanisms by DAC to inhibit the proliferation of cancerous cells is epigenetic pathway whereby decreased the gene expression of DNMTs in proliferating undifferentiated cells.