مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
The effect of the Acesulfame potassium on offspring spermatogenesis after treatment during pregnancy and prepuberty
The effect of the Acesulfame potassium on offspring spermatogenesis after treatment during pregnancy and prepuberty
Razieh Zereshki,1,*Ali Noori ,2Ali asghar Pilevarian,3
1. Department of Biology, Payame Noor University, Isfahan, Iran. 2. Departmentof Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran. 3. Department of Biology, Payame Noor University, Isfahan, Iran.
Introduction: Introduction: Considering the importance of nutrition, especially during pregnancy and prepuberty, and because of the prevalence of gestational diabetes as one of the diseases of this period, many mothers tend to use dietary products to prevent such problems. In this case, the mothers will have a sweet taste without calorie intake, while their tooth health will be maintained. Acesulfame potassium was discovered as one of the strongest sweeteners in 1967. It does not produce any calories in the body with a sweetening power, 200 times that of sucrose. Considering the increasing prevalence of these substances, it is necessary to investigate the effects of Acesulfame potassium. Therefore, in the present study, the effect of intraperitoneal injection of Acesulfame potassium during pregnancy was investigated on offspring spermatogenesis.
Methods: Materials and Methods: in this study, after maintaining virgin female rats weighted average 160 g in standard light and temperature conditions for two weeks, male rats were taken beside them and they were removed by observation of the vaginal plaque of the female rats and recorded on the first day of pregnancy. Then, a solution of Acesulfame potassium with four concentrations including 50, 100, 200 and 400 mg/kg were prepared and injected intraperitoneally to mothers during pregnancy A group was also considered as a control receiving 0.5 ml of normal saline daily. After lactation, intraperitoneal injection of Acesulfame potassium was started on male infants with the same previous doses and continued until puberty (3 months). After anesthesia with Ketamine, the animals were sacrifield and right testis was removed. The testes were placed in 10% formalin after weighing. Then, the tissue sections were prepared by staining with hematoxylin-eosin. Histopathological studies were performed on tissue sections morphologically as well as the number of spermatocyte, spermatid and spermatogonium cells in 10 fields of each lam. Finally, data was compared between different groups by ANOVA.
Results: Results: The histological results did not show any significant difference in the mean number of spermatogonium, spermatocyte and spermatid as well as testis ratio to body in comparison with the control group. Also, by studying the morphological status of seminiferous tubes, there were no significant disturbances in the spermatogenesis of the treated groups compared to the control group.
Conclusion: Conclusions: According to the findings, it seems that likely the use of Acesulfame Potassium during pregnancy and prepuberty does not pose a threat to spermatogenesis and testicular activity, although more extensive researches are required in this area.