• Genetic association of ADIPOQ gene variant rs822396 with type 2 diabetes
  • Seyedeh Parisa Chavoshi Tarzjani,1 Seyed Abolhassan Shahzadeh Fazeli,2,* Behnaz Hezarkhani,3 Zahra Sadeghi,4 Seyedeh Mohadese Shahzadeh Fazeli,5 Zahra Mirzayee,6
    1. Department of Genetic Faculty of Biological Sciences Tehran North Branch Islamic Azad University Tehran Iran
    2. Departments of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran and Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran
    3. Departments of Molecular and Cellular Biology Faculty of Basic Sciences and Advanced Technologies in Biology University of Science and Culture Tehran Iran
    4. Department of Genetic, Faculty of Biological Sciences, Tehran North Branch, Islamic Azad University, Tehran, Iran
    5. Alborz Medical University, Karaj, Iran
    6. Fazeli-Sanati Genetics Laboratory, Tehran, Iran


  • Introduction: Genetic and environmental factors influence serum adiponectin and may contribute to the risk of metabolic syndrome and type 2 diabetes (T2D). There have been many studies that have tried to investigate the association between different polymorphisms of the ADI¬POQ gene and T2D risk in all around the world. Therefore, in the present study, we investigated the association between adiponectin gene (ADIPOQ) polymorphism (rs822396), and the risk of T2D in the Iranian patients
  • Methods: For the present case-control study a total of 40 Patients with the T2D and 40 healthy individuals were selected for this study. The mean age at the time of collection of T2D patients was 58.5 years, in the control group at the time of collection was 48.5 years. T2D patients groups consisted of 18(45.0%) males and 22(55.0%) females and control group consisted 22 (55.0%) males and 18(45.0%) females. Whole blood was collected by venipuncture in tubes containing EDTA. Genomic DNA was extracted from peripheral blood using the NIGEB kit. The ADIPOQ gene polymorphisms (-3971A>G) were genotyped using DNA sequencing following PCR amplification. The forward and reverse primer sequence which was designed for the Single Nucleotide Polymorphism (SNP) using Perlprimer software, as follows: 5'TACAATCAGAGTCCGTTCTTGGTC3' (forward), 5'AGAAATGGGAAGAATCTGTGAGGC3' (reverse). The conditions for amplification were as follows: Initial melting step of 5 minutes at 95 ˚C, followed by 30 cycles of 50 second denaturation at 95 ˚C, 50 second annealing at 60 ˚C, 50 second extension at 72 ˚C, and a final elongation step of 5 minutes at 72 ˚C. The -3971A>G polymorphism in the ADIPOQ gene was performed by direct Sanger sequencing of the PCR products. The allele and genotype frequencies amongst cases and controls were compared by Chi-square test and P-value. Statistical analyses were performed using SPSS software. The Odds ratio (OR) with 95% confidence intervals (CI) were calculated to evaluate the strength of the association between examined ADIPOQ polymorphism and T2D.
  • Results: The results demonstrated that there were no significant differences in the distribution of genotypes and allele frequencies of ADIPOQ rs822396 between Iranian T2D patients and controls. (OR=1.215, P-value =0.43, X2=0.62)
  • Conclusion: Our data indicate that -3971 A/G polymorphism is not relevant in the susceptibility to or the severity of Iranian T2D patients. (P>0.05) Further large prospective studies are required to confirm these findings.
  • Keywords: Diabetes, ADIPOQ, Polymorphism, Iranian