• molecular study of c-Myc proto-oncogene gene dosage in patients with type 1 and type 2 diabetes
  • Asal Jalal abadi,1,* Seyed kazem Bidoki,2 Pegah Ghoraeian,3
    1. Department of genetics, Faculty of advanced sciences and technology, Tehran medical sciences, Islamic azad university, Tehran, Iran
    2. Gene afzar medical genetics center, karaj, Iran
    3. Department of Genetics, faculty of advanced sciences and technology, tehran medical sciences, islamic azad university, tehran, iran


  • Introduction: Diabetes is a common metabolic and multifactorial disease which categorized into two main types: diabetes type 1 and 2. type 1 diabetes is characterized by destruction of the pancreatic beta-cell; meanwhile, the type 2 diabetes occurs because of insulin receptor malfunction. Diabetes can lead to multiple complications such as neuropathy, nephropathy, etc. many people die from the disease every year. In this study, the effect of c-Myc gene dosage ( that is involved in various cancers) was investigated on diabetes.
  • Methods: The blood samples were collected from 30 healthy and 30 diabetic individuals (both type 1 and 2), then their DNA was extracted. Using dq PCR, a fragment of c-Myc gene was amplified and compared to an amplified fragment of y-IFN control gene. Subsequently, the PCR products were electrophoresed and the data obtained from the gel documenting machine was analyzed using UVitec software.
  • Results: According to the findings, the mean ratio of c-Myc to y-IFN (BIR) in healthy subjects, was 1.17295, the lowest and the highest BIR was 0.40298 and 2.27272 respectively. In contrast, in diabetic patients, the BIR mean was 1.28959 with the lowest BIR of 0.9975 and the highest BIR of 2.34736.
  • Conclusion: Our results showed that there is increase in gene dosage of c-Myc in diabetic patients in compare to healthy individuals, however there is no statistically significant difference between two groups of samples (Pvalue=0.225), so this oncogene may not have a role to trigger the disease in this limited iranian population.
  • Keywords: c-Myc proto-oncogene, diabetes, dq PCR, gene dosage