مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Investigation of synergic effect of Alfalfa extract and Lactobacillus rhamnosus on Blood lipid profile in diabetic male wistar rats
Investigation of synergic effect of Alfalfa extract and Lactobacillus rhamnosus on Blood lipid profile in diabetic male wistar rats
Atena Bazjoo,1,*
1. Department of Microbiology, Science Faculty, Islamic Azad University of Arak, Iran
Introduction: Among the most important metabolic diseases, diabetes
affects 190 million people worldwide, and is anticipated
to reach 300 million by 2025. Diabetes is a metabolic
disorder caused by reduced or lack of insulin secretion,
or insulin-resistant cells, which leads to increased blood
sugar and diabetic dyslipidemia
Recently, attention has been drawn to the role of microflora
in development of many diseases, including diabetes
Current studies on diabetes II address prevention or delaying
of this disease and its complications. Accordingly, it is highly
important to use foods with low glycemic index and with
bioactive agents such as probiotics
Probiotics are useful microorganisms, and in sufficient quantities
, show their benefits by improving intestinal microbiotic pattern.
This study aims to examine the effect of Lactobacillus rhamnosus as an indigenous Iranian probiotic on improving Blood lipid profile in
diabetic male Wistar rats
Methods: Thirty five- 4-week-old male Wistar rats, weighing approximately 110 to 120 grams were procured from Tehran Pasteur Institute, and housed in standard cages at 23±2 °C, in 12 hourly light/dark cycles, with free access to food and water.
After two weeks of adaptation, rats were randomly divided into 3 groups (7 each) as follows:
Negative control group (healthy rats), diabetic groups, probiotic group, including diabetic rats receiving 1ml PBS buffer containing 2× 109 cfu/ml of Lactobacillus rhamnosus. Diabetic groups receiving Alfalfa , group V: diabetic received probiotic and Alfalfa extract. Lactobacillus rhamnosus is used with concentration 109 cfu/ml .Probiotic and Alfalfa extract was given daily by gavage to groups
Diabetes was induced with intrapretoneal administration of 55mg/kg body weight of streptozotocin (Sigma Company).
Following streptozotocin injection, diabetes was confirmed in rats by measuring blood sugar.
Results: Results showed an increase in blood sugar, cholesterol, triglyceride, and LDL following the development of diabetes in rats, and a reduction in the number of lactic acid bacteria and yeasts, while the number of enteric bacteria increased. Use of probiotic or Alfalfa extract and combination of both was highly efficient in controlling blood sugar (maximum 62.2%). Combination of probiotic and Alfalfa extract significantly reduced cholesterol(59%), triglyceride, and LDL in diabetic rats.
Conclusion: Intestinal microflora including viable microorganisms count is 1014 cell/ml, belonging to 300 to 1000 different strains.
Recent studies have demonstrated that imbalance of microflora can lead to metabolic diseases such as obesity and diabetes .
This study examined the effect of Lactobacillus rhamnosus on diabetic male Wistar rats.
Results showed that probiotics can reduce blood sugar concentration in diabetic rats by 56.94%,It seems Lactobacillus rhamnosus consumes glucose as the main source of energy, and thus reduces serum blood glucose.
Cani et al. argue that increased intestinal gram-negative bacteria are associated with metabolic diseases, and proportions of intestinal clostridium and E. coli are substantially higher in diabetics .
Severe weight loss and thinning is a major problem in diabetics. In this study, significant weight loss was observed in rats following induced diabetes. Use of Lactobacillus rhamnosus prevented further weight loss in diabetic rats.The effect of probiotics on weight gain can be caused by production of digestive enzymes.
Probiotics have been shown to affect lipid profile with a number of mechanisms, including deconjugated bile acids, cholesterol absorption and assimilating cholesterol to stabilize membrane and cholesterol adhesion to cell wall of intestinal probiotics, conversion of cholesterol to coprostanol, inhibition of cholesterol synthesis from short-chained fatty acids in the liver such as probiotic-produced propionate or redistribution of cholesterol into liver