• Evaluation the effects of Fibromodulin protein expression on NFkB and TGFβ signaling pathways in liver cancer cells
  • Faezeh Ghasemi,1,*
    1. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


  • Introduction: Liver cancer is one of the most common cancers in the world and is considered the most lethal gastrointestinal cancer. fibromodulin is a main proteoglycan which contributes to remodeling of the ECM through binding to matrix molecules, thereby plays an essential role in tissue repair, tumor progression and cancer. The aim of this study was to investigate the effects of fibromodulin protein expression on NFkB and TGFβ signaling pathways in liver cancer cells.
  • Methods: First, fibromodulin was examined in cell culture media. It is then injected into the liver cancer model of the mouse. The level of gene expression is related to cancer as well as histological examination. Liver cancer cells were cultured. The fibromodulin gene is then introduced into the cell using lipofectamine, and the expression of the gene is measured using Real Time-PCR and protein production by Western blot and ELISA.
  • Results: There is showed increasing the concentration of the fibromodulin gene in cells, the amount of cell proliferation and growth increases. In this study, Western Blot method was used to evaluate the production of fibromodulin protein in the treated group. The results indicate that protein production in the treated group is significantly increased. (P <0.05). In this study, ELISA was used to evaluate the production of NFKB protein in the treated group. The results indicate that protein production in the treated group is significantly reduced. (P <0.05). The results of the NFKB route gene expression test showed a decrease in the treated group. The expression level of this gene was reduced compared to the reference gene and control group. Reduce the 1.5-fold reduction in gene expression.
  • Conclusion: The fibromodulin gene seems to have anticancer effects in the liver cancer model and can be used as an important therapeutic tool in liver cancer patients.
  • Keywords: Liver cancer, fibromodulin geneوNFkB and TGFβ signaling pathways