مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Normal, cancer skin cells refractive index extraction using Optical reflectance spectroscopy
Normal, cancer skin cells refractive index extraction using Optical reflectance spectroscopy
Afshan Shirkavand,1,*Ezeddin Mohajerani,2Leila Ataie Fashtami,3Mohamad hosein ghazi moradi,5
1. 1. Laser and Plasma Research Institute, Shahid Beheshti university , Tehran, Iran, 2. LASER medicine re Search group, MLRC, ACECR, Tehran, Iran, 2. Laser and Plasma Research Institute, Shahid Beheshti University, 3. Dermatology, Department of Regenerative Medicine, Royan Institute for Stem cell biology & Technology, ACECR, Tehran, Iran
Introduction: Background and objective: optical reflectance spectroscopy is a noninvasive technology for optical characterizing of biological samples. Any alteration in cell due to normal or carcinogenicity causes changes in refractive index. The aim of this study is to develop a new computerized program for refractive index extraction of some normal and cancerous skin cell lines such as melanoma, fibroblast, and adipose using Visible-Near IR reflectance spectrums and Kramers-Kroning relations.
Methods: Fiber optics based reflectance spectrometer (Ocean optics) in Vis-NIR wavelength, was applied for spectrum acquisition in an in-vitro study. Human skin cell lines including melanoma (A375), fibroblast and adipose (primary cultures) were cultured for optical spectroscopy. Following data acquisition, analytical MATLAB code based on KK relations was run. Validation of program is evaluated by three biological, liquid samples using Abbe Refractometry.
Results: validation error (below 5%), the determining of refractive index changes of cancer melanoma, and normal fibroblast skin cells in the wavelengths (450-950 nm) was done. The refractive index of melanoma is calculated 1.59270±0.0550 in 450nm; 321±0.0550 reached to a minimum amount of 1.27790±0.0550 in 620nm and showed a rough rise to 1.44321±0.0550 in 935 nm. The results for fibroblast cells are 1.33282±0.0134 in 450 nm with no considerable changes to 1.30494±0.0135 toward 935 nm.
Conclusion: Refractive index properties have shown potential in cancer screening and diagnosis. The result showed that optical spectroscopy is promising noninvasive tool for assessment of refractive index of biological living cells in study in-vitro study settings. By tracking refractive index changes in a non-invasive label-free method, there will be opportunity to screen normal and abnormal cells for probable alterations.