Introduction: Background and Aim: Diabetes mellitus is a disease which has affected about 3 million people in Iran. Formany years, the use of Phytotherapy in medicine has been common. In the present study, the effects of native probiotic on blood glucose s in diabetic rats were investigated.
Probiotics are living microorganisms that administration of sufficient quartiles of them cause health promotion. Since Probiotics modulate the composition of the intestinal microflora, they plays an important role in ameliorating the side effects of diabetes
Methods: Materials and Methods: For this study, 21 male wistar rats aged 12-14 weeks and weighed 120-140 gm was used. After a 1-week adaptation period,the rats were randomly divided into three group negative control, positive control (diabetic), trail 1 (probiotic) . Diabetes
was induced through the injection of stereptozotocin (55 mg/kg) into the experimental case and diabetic control .
Trail1 groupwas administrated with 2×109 cells of Lactobacillus gasseri by oral gavage .
After 21 days, rats were anesthetized with ether and blood samples were taken from heart.ThenGlucosekit is used for quantitative determination of Glucose in plasma
Results: Results:Ourresults showed that Glucose level in positive group (268±6.106mg/dl)was significantly increased compare to negative control(92.29±2.697 mg/dl),(Pvalue< 0.0001).
It also became clear that receiving probiotics in first trial group caused a significant decrease in Glucose concentration to(124.6±1.478mg/dl),(Pvalue< 0.0001).
Conclusion: Conclusion:The results of these tests indicate that the probiotic Lactobacillus gasseri as a probiotic withcumin extractcan reduce the negative effects of diabet and reduce levels of Glucose blood significantly.
Keywords: Key Words: Diabetes,Probiotic, glucose, rat