• Biofilm formation with microtiter plate 96 and pslA detection of P.aeruginosa isolates from clinical samples in Iran
  • Mohammad Reza Zolfaghari,1,* Mohammad Abootaleb,2 Nazila Arbab Soleimani,3 Nasim Ghorbanmehr,4 Mohammad Reza Yazdian,5
    1. Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran
    2. Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran
    3. Department of biology, Damghan Branch, Islamic Azad University, Damghan, Iran
    4. Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
    5. Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran


  • Introduction: Microorganisms attach to various surfaces and they have manufactured biofilms by production polysaccharides like PSL in P.aeruginosa. Synthesis of this kind of polysaccharide has done by PSL gene cluster. The aim of this study is consideration biofilm formation which is one of the major cause antibiotic resistance.
  • Methods: In this study, 100 P. aeruginosa were isolated with bacteriological and biochemical methods and they were tested for antibiotic-resistant testing as per CLSI guidelines. After that, pslA gene detection with PCR in all of the P. aeruginosa isolated from patients. Then, biofilm formation checked with microtiter plate method and it showed with SEM. Finally, expression of main attachment gene pslA in 6 strains could make moderate and strong biofilm were investigated by real-time PCR assay.
  • Results: In this study, 100 P. aeruginosa were isolated that these strains showed high rates of MDR. The presence pslA gene in all of the Pseudomonas isolated from patients was proven. Microtiter plate method showed 24 (24%) strains could make biofilm among 100 strains that showed with SEM. The pslA expression in strains which making moderate and strong biofilms are more than other strains.
  • Conclusion: Biofilms antibiotic resistance is one of the most important human problems for treating the bacterial infection, which one of the reasons can be the improper use of antibiotics and it is showing the motive to studying bacteria biofilms compared to the planktonic state. It is suggested, bacteria should investigate biofilm formation before antibiotics are prescribed. Hence, for bacterial biofilm treatment is recommended: before antibiotics are prescribed, biofilm formation by bacteria should be investigated.
  • Keywords: P. aeruginosa, microtiter plate, Biofilm, Real Time PCR, Scanning electron microscopy (SEM)