Effect of insulin on the proteins and apoptosis of hippocampus in male rats with diabetes mellitus type i

Roghayeh Abbasalipourkabir,1,* Giti kalantarian,2 Nasrin ziamajidi,3

1. Hamadan University of Medical Sciences
2. Hamadan University of Medical Sciences
3. Hamadan University of Medical Sciences



Insulin and insulin-like growth factor (igf) can prevent apoptosis in oligodendrocytes. the purpose of this study was to evaluate the effect of subcutaneous injection of insulin on the expression of igf gene and its proteins as well as of the apoptosis in hippocampus of streptozotocin-induced diabetic rats


An in vivo study was performed by subcutaneous injection of trade insulin in streptozotocin-induced diabetic rats. the duration of study was ten weeks and the diabetic rats was treated for two last weeks of the study. body weight, fasting blood glucose (fbs), expression of igf-i and igf-i genes and level of these proteins using real-time pcr and immunohistochemically (ihc) analysis were studied at the end of the experiment. hippocampal apoptosis was determined by tunnel assay.


The results of the present study showed the apoptotic effects of hyperglycemia in rat hippocampus. in addition, the in vivo results showed the efficacy of commercial insulin in compensated weight loss, fbs and protein levels of igf-1 and igf-2.


Although, the traditional insulin therapy could reduce apoptotic cells in the hippocampus, more studies could be considered to evaluate apoptotic signaling pathway. furthermore, behavioral study associated with memory and learning beside oxidative evaluation in hippocampus can reveal more details in future experiment.


Insulin، hippocampus، diabetes mellitus