In recent years, antibacterial peptides have been considered as an alternative for antibiotics because of raising resistance to antibiotics within pathogen's microorganism.
Methods
In this research, isolated bacteria from semnan were investigated for production of bacteriocin and endolysin. the supernatant from five strains was condensed by ammonium sulfate precipitation followed by purification using gf 75 superfine gel sephadex chromatography. ddbcc46 with the highest antibacterial activity (agar diffusion method) among the tested isolates was the candidate for current study. the plate containing dead escherichia coli and pseudomonas aeruginosa was tested with purified supernatant from ddbcc46.
Results
A 22 mm halo against both bacteria was detected, while using 60% ammonium sulfate purified antibacterial agent resulted in a diameter of 35 mm on the pseudomonas cell wall, proteus; 32 mm, k. pneumoniae, salmonella (sp) and e.coli 18 mm. two bands of 30 and 11 kda in sds –page was supposed to relate to this antibacterial agent. after purification by gel chromatography, bands of 30 kda and about 11 kda were collected and examined in diffusion agar method. their antibacterial activity was 40 mm for k. pneumoniae, 35 mm for p. aeruginosa and e.coli and 22 mm for salmonella (sp).
Conclusion
The bacteriocin isolated from semnan-iran; n70, showed a moderate antibacterial effects on the indicators bacteria.
Keywords
Antibacterial effects, bacteriocin, endolysin, gel chromatography
