Exotoxin gene examination in pseudomonas aeruginosa strains isolated from patients in sari hospital

Anahita Nejaty,1,* Hami kabousi,2 Fatemeh peyrouvi ghadikoulaei,3

1. Islamic Azad University of Ayatollah Amoli
2. Islamic Azad University of Ayatollah Amoli
3. Islamic Azad University of Qaemshahr Branch

Abstract


Introduction

Pseudomonas aeruginosa is one of the most opportunistic pathogens and as one of the main factors of hospital infection. the aim of this study was to detect the presence of the gene isolated in exos isolates from clinical samples.

Methods

: in this descriptive cross-sectional study, in 2017, sampling for 2 months, treatment centers, city of surrey. by performing biochemical diagnostic tests and the use of differential environments, on 40 samples (wound and urinary tract), 30 samples infected with pseudomonas aeruginosa. to check for the presence of pseudomonas aeruginosa isolates exos gene in isolation, pcr test was used.

Results

As a result of this investigation, the pcr test results showed that the existence of one of the most important factors gene exos angiogenesis, that is one of the most important factors are the existence of a creator of the disease pseudomonas aeruginosa isolates in all (100%) there were.

Conclusion

: according to the results of this study, there virulence exotoxin s factor can be a serious warning to the centers of disease control with the bacteria.

Keywords

Pseudomonas aeruginosa, hospital infections, exos gene, pcr