Evaluation of mir-210 and mir-874 expression in the patients infected with helicobacter pylori compared to gastric cancer patients using real-time pcr technique
: helicobacter pylori is a spiral-shaped, gram-negative, microaerophilic bacterium that selectively colonizes the gastric epithelium. it is proposed that a role of this bacterial effector in cell proliferation in tissue stomach and progressive of gastric cancer (gc). micrornas (mirnas) play key roles in regulating genes. the objective of this study is to evaluate the mir-874 and mir-210 expression in the patients infected helicobacter pylori compared with gastric cancer patients.
Methods
This cross-sectional study was performed on 40 stomach cancer patients with positive h.pylori and 40 positive h.pylori without stomach cancer and 12 negative h.pylori. expression of mirnas were evaluated using real-time pcr technique.
Results
The findings of the study indicated that changes of mirna-874 and 210 expressions in the blood of patients infected helicobacter pylori and gastric cancer are higher than healthy people who are also negative in terms of helicobacter pylori and gastric cancer.
Conclusion
: based on our results h.pylori but not stomach cancer can be expressed mir-210 and mirna874. it can be concluded that over expression of these mirnas is significantly associated with infection with h.pylori that this subject may play key roles in impaired apoptosis and progressive to cancer.
