Evaluation of nat8l expression in alzheime ,s rat model after hadscs intravenous administration
Mina Eftekharzadeh,
1,* Milad kazemiha,
2 Marjan shariatpanahi,
3 Arash sarveazad3,
4 Fatemeh moradi,
5 Hamidreza asgari,
6
1. Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran
2. Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran
3. Department of Toxicology & Pharmacology, School of Pharmacy, International Campus, Iran University of Medical Sciences, Tehran, Iran
6. Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran
Abstract
Introduction
In the present study, we transplanted human adipose-derived mesenchymal stem cells by intravenous injection in hippocampus of alzheimer,s disease (ad) rat model. the accumulation of β-amyloid peptide (aβ) and neuronal cell death in the brain play an important role in memory and learning dysfunction and cognitive impairment in alzheimer,s disease.the present study was designed to evaluate alteration of n-acetyltransferase 8 like (nat8l)
expression after intravenous administration of human adipose derived stem cells (hadscs) in
alzheimer,s disease (ad) rat model.
Methods
In this study, 32 male rats were
used in 4 groups: control, sham, ad rat model, and hadscs treatment
group. we used morris water maze (mwm) for evaluating behavioral changes
and western blot for assessing nat8l expression
Results
Behavioral
results showed that the spatial memory improved after hadscs injection in ad rat model.
consequently, there was also a significant increase in nat8l expression in hadscs treatment
group comparing to ad rat model.
Conclusion
According to our results in this study, the mechanism of
protective effects of hadscs in improving memory might be related to elevation of nat8l
expression.
Keywords
Alzheimer,s disease -hadscsc-nat8l-
