1. Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology 2. Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology 3. Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology 4. Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology
Abstract
Introduction
Cancer is the second leading cause of death all over the world that causes serious health problems. since chemotherapy produced a number of undesired and toxic side effects, natural therapies, such as the use of plant-derived products in the treatment of cancer, may reduce adverse and toxic side effects(bournine et al., 2017). peganum harmala l. as a medicinal plant that commonly used in folk medicine, includes phyto-chemical molecules such as quinazoline alkaloids, beta-carboline alkaloids and flavonoids(wen et al., 2014). it has gained great interest due to its important anti tumoral properties.
Methods
In this study we aimed to extract and characterize the active component of peganum harmala’s seed via ftir and lcmss, as well as to evaluate the in vitro anticancer effect of its extract against two different human breast cancer cell lines: mcf-7 cells and mda-mb-231 cells.
Results
Results showed that the method of extraction could isolate alkaloids with high purity and the main alkaloid that detected by lc-ms was harmine which is the main betacarboline intercalates through the dna strands and suppresses topoisomerase enzymes. mtt assays represent that 30 μg.ml-1 of extract could inhibit cell growth of both mcf-7 cells and mda-mb-231 cells in a 24h treatment.
Conclusion
Acid-base extraction of p. harmala’s seeds alkaloids, demonstrated anti-tumoral impact on both breast cancer lines, confirming its anti-cancerous properties.
