1. Medical Biotechnology Department, Biotechnology Research Center, Semnan University of Medical Sciences 2. Medical Biotechnology Department, Biotechnology Research Center, Semnan University of Medical Sciences
Miniature organs called organoids are three-dimensional cell cultures of stem cells or induced pluripotent stem cells from various organs such as intestine, liver, brain, kidney, stomach and retina that can model main attributes of whole organs. in organoid systems for organizing a large range of tissue types require only a small number of cells.
This is a review article.
Current technical advances in developing the organoids of the intestine and liver have shown the practical potential of organoid systems as a valuable tool for employing in biomedical researches such as the modeling of disease, examing organ development and tissue morphogenesis, testing cytotoxicity for drug screening and developing organoid-based treatments including regenerative and personalized medicine. in addition, apart from the above-mentioned, the organoid technologies can also be used for developing cancer organoids in order to model cancer development and treatments.
In spite of the enormous applications of organoid-based technologies, they have important limitations that must to be overcome. in this paper, we will present a comprehensive review on recent advances in the development of organoid-based systems and their applications in biomedical researches. also there is a particular focus on main limitations of these systems.