1. Department of Toxicology & Pharmacology, School of Pharmacy, International Campus, Iran University of Medical Sciences, Tehran, Iran 2. Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran
Abstract
Introduction
Alzheimers disease (ad) is a progressive neuropsychiatry disorder that gradually deteriorates memory and behavioral functions. it seems that there is a correlation between necroptosis and various neurodegenerative disorders. the present study was designed to assess rip-1, rip-3 expression as necroptotic factors after intravenous administration of human adipose derived stem cells (hadscs) in alzheimers disease (ad) rat model.
Methods
Thirty-two male rats were used in 4 groups; control, sham, ad rat model, and hadscs treatment group. the hadscs characterization was confirmed by flowcytometry technique. thioflavin s was utilized for detecting aβ plaques in ad rat model following injection of hadscs. we used immunofluorescent method for evaluating rip-1 and rip-3 expression.
Results
Statistical analysis revealed that thio-s positive plaques number had a significant increase in ad rats while administration of hadscs significantly decreased thio-s positive plaques number in ad group (###p-value<0.001).
we found for the first time that the expression of rip-1and rip-3 increased significantly in ad rat model comparing to the control group and decreased significantly in hadscs treatment group comparing to ad rat model.
Conclusion
According to our results in this study, the mechanism of protective effects of hadscs in ad might be related to reduction of necroptotic markers.
