Evaluation of dha induced survivin gene expression in colorectal cancer cells
Farideh Hosseini samadyoorddi,
1,* Mohammadreza sam,
2 Nasrollah jabbari,
3 Hossein mozdarani,
4
1. Department of Radiology Technology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2. Department of Cellular and Molecular Biotechnology, Institute of Biotechnology, Urmia University, Urmia, Iran.
3. Department of Medical Physics and Imaging, Solid Tumor Research Center, Urmia University of Medical Sciences, Urmia, Iran
4. Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Abstract
Introduction
Survivin is one of the iaps that inhibits apoptosis. over expression of survivin gene as a landmark of malignant cells is known as a resistant factor against to cancer therapy agents too. then finding the safe, inexpensive and attainable compounds that suppress survivin gene expression and cooperated with radio-chemo therapy agents can increase cancer therapy efficacy and diminish its side effects. in this study we evaluated the effect of omega-3 docosahexaenoic acid (dha) on surnivin gene expression in colorectal cancer cells (crc) as a radio-sensitizer agent.
Methods
Radioresistant ht-29 cells were pretreated with 50 and 100 µm dha before exposing to 2 and 10 gy of gamma radiation. after 48 hr survivin mrna was extracted then we compared survivin mrna level in treated gropes with each other.
Results
Dha reduced survivin gene expression level singly or in combination with radiation, whiles we don’t have significant reduction in gropes that only take gamma ray.
Conclusion
Survivin gene expression was resistant to radiation. dha can be considered as a radio-sensitizer agent in crc by mechanism of survivin gene suppressing and increasing radiation induced apoptosis
Keywords
Survivin, dha, crc. radiation,
