Changes in expression of xiap anti apoptotic gene in mcf-7 cell category of breast cancer after 24 hours treatment with cyclophosphamide

Somayeh Gholami,1,* Dr.golnaz asaadi tehrani,2 Narges mohammadi,3 Fateme modhejiyan,4

1. Department of Genetic group, Zanjan Branch, Islamic University, Zanjan,Iran
2. Department of Genetic group, Zanjan Branch, Islamic University, Zanjan,Iran
3. Department of Genetic group, Zanjan Branch, Islamic University, Zanjan,Iran
4. Department of Genetic group, Zanjan Branch, Islamic University, Zanjan,Iran

Abstract

Introduction

Breast cancer has become a serious problem for human societies in recent fifty years. data shows that approximately one out of nine women experiences breast cancer during their lifetime. breast cancer occurs as a result of disorders in key genes. xiap is one of these key genes that has a great influence in breast cancer. xiap is a human x chromosome dependent, apoptosis controlling protein and as an internal caspide controlling factor, xiap inhibits caspases 3, 7 and 9 and any functional defection or disorder in these proteins can result in cancer, neurodegenerative diseases and self-immunity disorders, it also plays an important role in regulation of intracellular signaling and accelerating the reaction of receivers. cyclophosphamide is a dna replication and an anti neoplasm alkylation inhibitor and is one of the most common medicines used for treatment of cancer. this study deals with genetic changes in order to help diagnose and treat breast cancer.

Methods

For this propose, were prepared cell category of human breast cancer (mcf-7) from cellular bank of pastor institute and were treated with cyclophosphamide at concentrations of 1, 5 and 10 µm after counting and passage. roche and takara kits were used respectively for rna extraction and cdna synthesis and optimize the situation of real time pcr was used to estimate effectiveness of the medicine in changing expression of xiap after 24 hours of treatment and finally the data was analyzed using the rest software.

Results

Our study showed that xiap is an anti apoptotic gene and the more it is expressed the worse breast cancer gets; the results showed that after 24 hours of treatment with cyclophosphamide, this factor resisted against this medicine (p=1) and did not have any positive influence in decreasing xiap expression and death of cancer cells.

Conclusion

The studies showed that taxol regulates xiap and decreases its expression and that is how it regulates breast cancer cells by regulating their apoptosis. but our results showed that 24 hours of treatment with cyclophosphamide does not decrease expression of xiap and apoptosis in breast cancer cells.

Keywords

Xiap, breast cancer, cyclophosphamide, rest