Antidermatophyte effect of stachys schtschegleevii essential oil on trichophyton rubrum (ptcc 5143)

Shiva Mirfattah,1,* Behin omidi,2 Arezoo dastpak,3

1. Islamic Azad University, Central Branch, Tehran, Iran
2. Assistant professor of Biology Group, Islamic Azad University, Central Branch, Tehran, Iran
3. Assistant professor of Biology Group, Islamic Azad University, Central Branch, Tehran, Iran

Abstract

Introduction

Trichophyton rubrum is one of the most common dermatophytes that causes dermatophytosis in the world and iran, it can cause infection in the skin, hair and nails. according to epidemiological reports, fungal diseases are on the rise and on the other hand, drug resistance has also been observed, and the fungal drugs that are used today have high side effects so researchers are looking for new drug for the treatment and control of dermatophytosis infections. plants have a high potential for influencing pathogenic microorganisms, so in this study we used stachys schtschegleevii essential oil against trichophyton rubrum (ptcc 5143) .

Methods

Stachys schtschegleevii plant was collected from a habitat around tabriz city and then confirmed by herbarium of jahad agriculture. trichophyton rubrum (ptcc 5143) was used in this study were prepared from fungal colocation of tehran university then cultured in scc and incubated in 280c for 7-14 day. the essential oil of the stachys schtschegleevii was taken by the clevenger and then kept it in dark glass .َantifungal effect of them was measured by disc diffusion method , mic80 and mfc.

Results

: the results showed that the diameter of the inhibition zone of the stachys schtschegleevii essential oil was 22.47±1 mm which is not significantly different from that of griseofulvin)p≤0.05). the concentration of essential oil obtained as a mic80 has been 3.125±0.5mg/ml and the concentration of essential oil obtained as a mfc has been 1.56±0.85mg/ml which is not significantly different from that of griseofulvin, nystatin and terbinafine (p≤0.05).

Conclusion

: according to the results, the essential oil of stachys schtschegleevii has a high antifungal effect potential on trichophyton rubrum. as a result, the essential oil of plants are a very suitable and safe substitute for the treatment of fungal diseases such as dermatophytosis.

Keywords

Trichophyton rubrum, stachys schtschegleevii, dermatophyte