Antibacterial studies of synthesized titanium oxide nanoparticles

Elham Abdolmajid,1,* Fahimeh nemati,2 Mojtaba falahati,3

1. Department of Biotechnology, Faculty of Advance Science and Technology, Tehran Medical Sciences, Islamic Azad University (IAUPS)
2. Department of Biotechnology, Faculty of Advance Science and Technology, Tehran Medical Sciences, Islamic Azad University (IAUPS)
3. Department of Nanotechnology, Faculty of Advance Science and Technology, Tehran Medical Sciences, Islamic Azad University (IAUPS)

Abstract

Introduction

In recent years it has been increasing attention on designing and development of new and more potent antibacterial particles due to the appearance of drug resistant bacterial strains. particularly, tio2nps have been extensively introduced as effective antibacterial agents in the last decade. in this regard, this study is focused on the fabrication of tio2 nps by sol-gel method and exploring their antibacterial activities against pseudomonas aeruginosa; escherichia coli.

Methods

Tio2 nps were synthesized by sol-gel route. to determine the antimicrobial activity of fabricated tio2 nps, the “well diffusion method” was carried out using pseudomonas aeruginosa (atcc 27853); escherichia coli (atcc 25922) and staphylococcus aureus (atcc 25923). the minimum inhibitory and minimum bactericidal concentrations of tio2 nps were determined against bacterial strains by micro dilution method.

Results

Tio2 nps showed a nano-sized distribiution of around 30 nm with strong antibacterial affect against e.coli, p. aeruginosaand s. aureus with mic= 50±0.77, mic═50±0.80 µg/ml, and mic═25±1.77µg/ml, respectively. the mbc observed in the present study for synthesized tio2 nps was50±0.57μg /ml, 50±0.50 μg /ml and 25±1.77 μg /ml for e. coli, p.aeruginosa and s.aureus, respectively.

Conclusion

The mic of tio2 nps against e.coli, p. aeruginosa and s. aureus were reported to be 9.7µg/ml,19µg/ml and19.5µg/ml, respectively. it seems that the difference between the results of the present study and other reported data is due to different colloidal stability and size distribution of nps. in conclusion, the fabricated tio2 nps pronounced antimicrobial activity against both gram-negative and gram-positive bacteria. the synthesized tio2 nps show great potential to be utilized as antimicrobial nanomaterials against microorganisms.

Keywords

Titanium oxide nanoparticles, fabrication, antibacterial