Multiple antibiotic resistance patterns of the most common isolates from different form of meat
,1 Nima bahador
1. Department of microbiology, College of Science, Shiraz Branch, Islamic Azad university, Shiraz, Iran
2. Department of microbiology, College of Science, Shiraz Branch, Islamic Azad university, Shiraz, Iran
The extensive use of antibiotics in the breeding of food-producing animals has led to an increase in drug-resistant pathogens. although these pathogens could be transmitted to humans in different ways, therefore, identification and determination of their antibiotic pattern could be considered as a good solution. in this regard, the study tried to isolate common pathogenic bacteria from different types of meat and then evaluate their antibiotic susceptibility.
In this research, 50 meat samples including: whole meat, minced meat and fish were purchased from different areas of shiraz city. the samples were transferred to the lab and cultivated in nutrient broth as well as blood agar. then the isolates were purified and the most common isolates identified using gram staining, catalase, oxidase, growth ontsi and imvic tests. furthermore, antibiotic pattern was evaluated using kirby-baure method according to clsi 2017 standard versus 16 antibiotics and the data were analyzed using anova- duncan statistical tests. finally the most common isolates were identified by molecular technique.
Although different types of bacteria were isolated from meats and they were identified using biochemical tests and confirmed using molecular technique as hafnia alvei, enterobacter cloacae, escherichia coli and aeromonas salmonicida, the most common isolates was hafnia alvei. furthermore, the isolates showed different antibiotic resistance pattern and according to the results they were considered as multi drug resistant bacterium. statistical analysis carried out revealed not significant differences in respect to the isolates and antibiotics.
In general, the results of this study showed that among common species hafnia alvei with (50%) frequency was the most common isolates with multi drug resistance.
Antibiotic resistance, meat, hafnia alvei