Comparison between local and systemic administration of msc-derived cm on ameliorating ova-inducted asthmatic changes in rats

Mahdi Ahmadi,1,*

1. Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences



Today, stem cell therapy has opened a promising therapeutic strategy for asthma. selecting msc-derived conditioned media (cm) instead of stem cells, could circumvents some of the pitfalls of mscs such as risk of developing cancer, xenozootic contaminations and total costs. our objective was to compare the potential therapeutic effects of msc-derived cm after either local or systemic administration on ameliorating ova-inducted asthmatic changes.


28 male wistar rats (200-250 g, 6–8 weeks old) were enrolled to the current study. four healthy rats were blindly used for isolation of rat bone marrow-derived mscs (rbmmscs) and conditioned media (cm) harvesting. the rest of 24 animals were classified into four experimental groups (n=6 for each group); control rats (c group), sensitized rats (s group), sensitized rats received intratracheally 50 μl cm (st+cm group) and sensitized rats received intravenously 50 μl cm (sv+cm group). rats in the sensitized groups were actively exposed to ova over a period of 32 ± 1 days. in the control group, normal saline solution was applied instead of ova. one-day post sensitization (day 33), sv+cm group and st+cm group received cm intratracheally and intravenously, respectively. two weeks post treatment with cm (day 45); the expression of interleukin (il)-4, il-13, and il-10 in lung tissues of asthmatic rats was measured by real-time pcr.


Our data indicated a marked expression in the levels of il-4 and il-13 with decrease of il-10 in asthmatic groups as compared to healthy rats (p < 0.001 to p < 0.01). the expression of measured mrna in st+cm group showed drastic difference compared with other sensitized groups. in detail, the expression levels of il-4 and il-13 diminished significantly (p<0.01), whereas il-10 increased simultaneously (p<0.05). in addition, the pattern of il4, il-13 and il-10 expression in sv+cm group were more similar to group s.


The results of this study showed direct administration of cm could be effective in alleviation of asthmatic changes. however, no significant modulatory effects originating from systemic administration of cm were also evident in asthmatic rats.


Asthma, msc-derived cm, intravenously, intratracheally