Green synthesis of zince oxide nanoparticle for evaluation cytotoxicity on dental pulp cells

Faezeh Keshavarzian,1,* Ali niapour,2 Aziz habibi-yangjeh,3 Mohsen arzanlou,4 Hadi peeridogaheh,5



Due to the increasing use of nanoparticles, researchers have devised a novel method to eliminate the disadvantages of previous procedure called green synthesis.the aim of this study was synthesis zinc oxide nanoparticle from nontoxic and biocompatible chemicals and to assess its cytotoxicity


To make zinc oxide nanoparticle , zinc nitrate hexahydrate is used as a zinc source as well as water is used as solvent.naoh (0.2 m) was used as reducer. the solvent was sonicated for 60 min.preparing of pulp cells: tissue pulp were extracted. it was breaked into small pieces. collagenase was used to tissue digestion and for 6 hours was placed in the incubator. dental pulp cells were treated with a wide range of nanoparticle concentrations (1,2,4,8,16,32,64,128,256,512,1024µg/ml).after 24 hours ,mtt test was performed. for this test , the 180 µg of culture medium without fbs and the 20 µg of mtt solution (0.5 µg/ml) was added to each well.after 4 hours, the cell culture medium was removed and 200 µg dmso was added to the wells, and after the 10 min styling of its attraction was read by eliza reader


The survival rate of dental pulp cells did not decrease to 16 µg/ml concentration of zinc oxide nanoparticle and cytotoxicity detected in 32 µg/ml concentration of zinc oxide nanoparticle


The green synthesized zinc oxide nanoparticles can be used in low concentration in medicine


Green synthesis , pulp cells , zinc oxide nanoparticle, cytotoxicity