Effect of curcumin based electrospun nanofibrous scaffolds on proliferation and stemness preservation of adipose derived stem cells
,1 Mohammad pourhassan-moghaddam
,2,* Maryam ghobeh
1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2. Department of Medical Biotechnology, Tabriz University of Medical Sciences, Tabriz ,Iran
3. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
An ideal biomaterial in regenerative medicine should be able to regulate the stem cell proliferation without the loss of its pluripotency. curcumin (cur) is a naturally occurring ﬂavone with a wide spectrum of biological functions including anti-inﬂammatory and anti-oxidant properties. the present study describes the inﬂuence of cur-loaded nanoﬁbrous mats on the regulation of proliferation and stemness preservation of adipose-derived stem cells (adscs).
For this purpose, cur-loaded poly (e-caprolactone)/poly (ethylene glycol) (pcl/peg) nanoﬁbrous mats were produced via electrospinning process and the successful fabrication of these bioactive mats was conﬁrmed by ﬁeld emission scanning electron microscopy (fe-sem) and fourier transform infrared spectroscopy. adscs were seeded on the nanoﬁbers and their morphology, viability, and stemness expression were analyzed using fe-sem, mtt, and qpcr assays after 2 weeks of incubation, respectively.
The results display that adscs exhibit better adhesion and signiﬁcantly increased viability on the chr loaded pcl/peg nanoﬁbrous mats in relative to the pcl/peg nanoﬁbers and tissue culture polystyrene. the greater viability of adscs on cur based nanoﬁbers was further conﬁrmed by higher expression levels of stemness markers sox-2, nanog, oct-4, and rex-1.
These ﬁndings demonstrate that cur-loaded pcl/peg electrospun nanoﬁbrous mats can be applied to improve cell adhesion and proliferation while concurrently preserving the stemness of adscs, thus representing a hopeful potential for application in stem cell therapy strategies.
Curcumin, electrospun nanoﬁbers, stem cells, pluripotency