Diagnosis and determination of chemotherapy in an in vitro environment

Amirhossein Alimohammadian,1,* Negin maleki,2 Faezeh sarvar,3 Afsaneh bazgir,4 Zahra pakzad,5

1. Member of the brilliant talent club of young and elite scholars
2. Member of the brilliant talent of young and elite peoples youth club of Ardabil Islamic Azad University, Iran
3. Department of Immunology
4. Department of Genetics
5. Department of Genetics

Abstract


Introduction

One of the ways to treat cancer is chemotherapy. chemotherapy drugs are basically based on inhibiting the cell cycle, inhibiting metastatic signaling inhibition. chemotherapy drugs are always prescribed by the physician's individual experiences and observations, and the time passed in malignant tumors is irreparable. also, the dosage of lung cancer drugs has been documented through flupple tubes, which is similar to that.

Methods

The loading of the drug onto the cancerous cell and the tissue samples in a laboratory environment on supplemented racks containing the cancerous cells that were dissected, and after cell proliferation and treatments on the cells, the readings are performed through elisa reader and using the amount of information absorption is obtained, such as the amount of inhibition of cancerous cells.

Results

With this method it is possible to diagnose the effect of the drug. this method makes the chemotherapy personalization of each patient based on its genetic and immunological characteristics and monitoring the stages of treatment, predicting and controlling the possible complications and prescribing appropriate drugs and, most importantly, avoiding waste of time for the continuation of treatment.

Conclusion

Converting tissue to the cell for testing or directly using it and performing a live test using spectrophotometric method. the type of effective drug also reports the dosage to the physician.

Keywords

Chemotherapy-tissue biopsy-elisa