Relation between polymorphism rs2227284 of il4 and prostate cancer

Neda Khayat hesari,1 Zahra tahmasebi fard,2,* Iman salahshouri far,3

1. Department of biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2. Department of Biology, Roudehen Branch, Islamic Azad of university, Roudehen, IRAN
3. Department of biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Abstract

Introduction

Inflammation in tissues is one of the cancer factors. interleukins secreted by t helper cells like il4 can have notable impacts on cancer patients. this interleukin with its dual role, in early stages of prostate cancer, prevents the progression of the tumor but in late stages facilitates the growth. in this research, the relationship between polymorphism of rs2227284 (-33c/t) in the il4 gene and prostate cancer incidence was deliberated.

Methods

100 blood samples from prostate cancer patients and 100 samples from healthy people in the same range of ages were randomly selected under the supervision of a specialist physician. dna samples were extracted by salting out method and their purity did examine and proved by spectrophotometry and electrophoresis. then using appropriate primers the selected sequence was multiplied and digested with specific enzyme nla iv. results obtained were processed by spss software, statistic x2 calculations, logistic regression and …

Results

Mutant genotype cc in rs2227284 polymorphism showed a meaningful relation between patients and control samples (p-value: 0.004, or: 2.31, ci95%: 1.309-4.077) chance of developing prostate cancer was 2.31 times more than other genotypes. this genetic change showed no meaningful statistic relation with age (p-value: 0.063) body mass index (p-value:0.732) cancer grade (p-value:0.570) and histopathologic chances (p-value:0.277).

Conclusion

Genetic change from t to c in the location 132677033(nt) of the il4 gene is seemingly related to prostate cancer occurrence in iranian patients.

Keywords

Rs2227284, prostate cancer, rflp-pcr