Axon elongation of differentiated motor neuron-like cells from human endometrial stem cells by microtubule stabilizer drug

Narges Mahmoodi,1 Jafar ai,2 Somayeh ebrahimi-barough,3 Zahra hassannejad,4 Elham hasanzadeh,5 Vafa rahimi-movaghar,6,*

1. Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences
2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences
3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences
4. Pediatric Urology and Regenerative Medicine Research Center, Tehran University of Medical Sciences
5. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences
6. Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences

Abstract

Introduction

Epothilone b (epo b) is a microtubule stabilizing agent that recently is mentioned to promote axon regeneration of damaged axons of mature neurons after spinal cord injury. the aim of this study is to evaluate the effect of epo b on axon elongation of motor neurons differentiated from human endometrial stem cells (henscs).

Methods

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) test was used to assess cell viability. the axon elongation in motor neuron-like cells differentiated from human endometrial stem cells was evaluated by immunofluorescence (if) using beta tubulin (tuj 1) marker to show the whole cell morphology.

Results

The mtt test did not show any cytotoxicity on henscs (epo b doses less than 5 nm). the results of if of differentiation group with 10 nm epo b showed more axon elongation compared to the differentiation group without using epo b. the if also showed brighter expression of beta tubulin under the fluorescence microscopy.

Conclusion

The eop b promotes axon elongation during motor neuron differentiation of henscs.

Keywords

Axon elongation, epothilone b, endometrial stem cells