Antidermatophyte effect of olea europaea leaf extract on trichophyton mentagrophytes

Parya Roghani,1,* Arezoo dastpaak,2

1. Department of Biology, Faculty of sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2. Department of Biology, Faculty of sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran

Abstract

Introduction

Dermatophytosis is a common fungal disease that caused by dermatophytes. dermatophytes are of keratin friendly fungi that involve keratinized tissues such as hair, nail and skin. in recent years, increased antifungal drug resistance was reported, in addition, antifungal drugs have limited effects and have many side effects. for example griseofulvin has been available antifungal agent for the treatment of dermatophytosis for about 40 years, but it has side effects such as nausea, diarrhea, headache, rash, and sometimes allergy, ketoconazole causes the inhibition of liver enzymes and increase in the concentration of other drugs, and inhibition of synthesis of steroid hormones. recently scientists interested to treatment infection diseases by herbs. herbals are effective alternatives for chemical treatment methods. in this research, the antidermatophyte effect of olea europaea leaf extract on trichophyton mentagrophytes (zoophilic dermatophyte),was studied.

Methods

We collected olive leaves and dried in shade then put them in powder form and extracts of olea europaea leaf were extracted using soxhlet. trichophyton mentagrophytes (ptcc 50541) was used in this study were prepared from fungal colocation of tehran university then cultured in scc and incubated in 280c for 7-14 day. antifungal effect of them was measured by disc diffusion method , mic80 and mfc.

Results

The results showed that the diameter of the inhibition zone of the olea europaea leaf extract was 24±0.5 mm which is not significantly different from that of griseofulvin)p≤0.05). the concentration of extract obtained as a mic80 has been 0.78±1mg/ml and the concentration of extract obtained as a mfc has been 0.39±1.2 mg/ml which is not significantly different from that of griseofulvin, nystatin and terbinafine (p≤0.05).

Conclusion

According to the results, the olea europaea leaf extract has a high antifungal effect potential on trichophyton mentagrophytes. as a result, the extract of plants are a very suitable and safe substitute for the treatment of fungal diseases such as dermatophytosis.

Keywords

Dermatophytes, dermatophytosis, olea europaea, trichophyton mentagrophytes