Genotyping of human papillomavirus in cervical samples with cancerous and precancerous diagnosis

Shabnam Nemati,1,* Masume aslanimehr,2 Taghi naserpour-farivar,3 Fatemeh samiee-rad,4 Dariush hamedi asl,5

1. Department of Microbiology, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
2. Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
3. Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
4. Department of pathology, Qazvin University of Medical Sciences, Qazvin, Iran
5. Department of Molecular Medicine, Faculty of Medical Sciences, Qazvin University of Medical Sciences, Qazvin, Iran

Abstract

Introduction

Cervical cancer is the second most incident cancer in women especially in developing countries and also one of the main leading causes of women’s death worldwide. considering the high incidence and geographical distribution of human papillomaviruses and the correlation between hpv and cervical cancer, genotyping of high-risk human papillomaviruses in precancerous and cancerous patients by real-time pcr is the aim of this study.

Methods

totally, non-duplicated 111 formalin-fixed paraffin-embedded cervical samples during 1999-2015 were obtained. for primary detection of hpv genome, first, dna of biopsies were extracted, then samples subjected to real time pcr by using one type of specific primers for hpv16, 18, 31, 33, 45, and 52.

Results

Hpv genome were detected in 92.23% of samples. according to hpv genotyping data, generally, 67.36% of samples were infected by hpv16, 49.47% were infected by hpv45. generally, 77.89% of samples were reported as multiple hpv infection.

Conclusion

Our analysis revealed high incidences of hpv16 and other high risk types among cervical disorders and malignancies. our data are suggesting that the vaccine programs and prevention strategies should be developed and improved.

Keywords

Cervical cancer, high-risk human papillomavirus, genotyping, real-time pcr