1. Islamic Azad University, Tehran, Iran. 2. Malek Ashtar University of Technology, Tehran, Iran. 3. Islamic Azad University, Tehran, Iran. 4. Malek Ashtar University of Technology, Tehran, Iran.
vibrio cholerae is one of the most important intestinal and waterborne pathogens that have high prevalence in world. traditional methods for v.cholerae detection are time consuming and laborious with low sensitivity. herein, a localized surface plasmon resonance (lspr) nanobioprobe has been developed based on specific immunological interactions between gold nanoparticles (gnps) conjugated with antibody and v.cholerae in order for the rapid detection.
In this research, plasmonic gnps were conjugated to antibodies and the conjugation confirmed using uv/vis spectrophotometer and dynamic light scattering (dls). after that, the sensitivity of the nanobioprobe has been investigated in the presence of different concentration of v.cholerae via measuring the lspr band shifts of gnps. also lspr sensitivity of nanobioprobe was compared with the elisa method.
The data was showed; the lspr biosensor reduced the v.cholerae detection time from 2 or 3 days to less than 1 h compared with traditional methods.
In conclusion, this nano-biosensor detection can be used like a simple diagnostic test in clinical laboratories for identification of v. cholerae.