A green approach for synthesis of selenium-based nanoparticles with antimicrobial activity

Farnoush Asghari,1,* Mohammad imani,2 Mehdi razzaghi-abyaneh,3 Hashem rafii-tabar,4

1. Shahid Beheshti University of Medical Science
2. Iran Polymer and Petrochemical Institute
3. Pasteur Institute of Iran
4. Shahid Beheshti University of Medical Science



The association of selenium and sulfur makes a magic combination with antifungal features of both selenium and sulfur. typically selenium sulfide is prepared through hazardous chemical ways. we synthesized selenium sulfide nps in a green approach for the first time and examined their properties in vitro.


Fusarium oxysporum was used as a bio-factory which was confronted with selenous acid (3 mm). the changes observed 48 h after adding 5-day fungal elements into the selenous acid solution and incubation at 30c with shaking at 180 rpm the emergence of nps was proved and determined by uv-vis spectrophotometry, dynamic light scattering (dls), scanning electron microscopy, energy-dispersive x-ray spectroscopy (edx), x-ray diffraction (xrd) and fourier-transform infrared spectroscopy (ftir) techniques.


Uv-vis spectrophotometry showed the color change obtained from nps synthesis quantitatively. it demonstrated a maximum absorbance at 217 nm for the test. an absorption in the range of 200 to 220 nm is attributed to sulfide and thiols which was the first step in approving the existence of selenium sulfide nps. in sem micrographs nps were seen encompassed in bio-mass of the medium and cells debris with a diameter of 34.32 to 231.98 nm. dls analysis showed the average hydrodynamic diameter of the obtained nps 81.9 nm. in edx analysis the existence of selenium and sulfur spectral peaks of the nps structure was proved. in ftir spectra, the peaks at 1090-1020 and 1650-1580 cm-1 and also at 1580-1490 cm-1 verified the presence of primary and secondary amine band. also sulfide compounds attributed peaks were obtained around 600 to 650 and also 1550 cm-1. the xrd pattern which was taken of the nps after 48 h reactions had the strongest bragg’s reflection with the closest match with ses, ses2 and se according to standard jcpds cards 00-002-0320, 00-047-1481 and 00-042-1425 respectively.


Selenium and selenium sulfide nps, with an improved antifungal activity, are achievable through a green safe biosynthesis approach by f. oxysporum.


Fusarium oxysporum, selenium, nanoparticles, green synthesis