Nrf1 and nrf2 knockdown effect in mitochondrial biogenesis

Mahshid Shabanpour,1 Raana sirous azar,2 Solmaz khalifeh,3,*

1. Amir-Almomenin Hospital, Tehran Medical Sciences, Islamic Azad University
2. Cognitive and Neuroscience Research Center (CNRC), Tehran Medical Sciences, Islamic Azad University
3. Cognitive and Neuroscience Research Center (CNRC), Tehran Medical Sciences, Islamic Azad University



The transcription factor nf-e2 p45-related factor 2 (nrf2; gene name nfe2l2) allows adaptation and survival under conditions of stress by regulating the gene expression of diverse networks of cytoprotective proteins, including antioxidant, anti-inflammatory, and detoxification enzymes as well as proteins that assist in the repair or removal of damaged macromolecules. nuclear erythroid 2-related factor 1 (nrf1) stand as an important regulator of the adaptive response to oxidants and play important role in coordinating antioxidant defense system


In current study small interfering rna (sirna) targeting nrf1and nrf2 (nrf1&2) was injected in dorsal third ventricle of adult male albino wistar rats. next, we used western blot analysis to evaluate the protein level of mitochondrial biogenesis factor.


Based on our findings, nrf1&2- silenced rats induced anxiety-like behavior compared to the control group. the level of peroxisome proliferator-activated receptor gamma coactivator-1 (pgc-1) protein increased in hippocampus, prefrontal cortex and amygdala regions.


Present data evaluated the complexity of mitochondrial functions and nrf1 and nrf2 in rat’s brain in addition to its effect on pgc-1 level also nrf2 has a crucial role in the maintenance of cellular redox homeostasis by regulating the biosynthesis, utilization, and regeneration of glutathione, thioredoxin, and nadph and by controlling the production of reactive oxygen species by mitochondria and nadph oxidase.


Nrf1, nrf2, rat, mitochondia