Antibacterial evaluation of imipenem/sulbactam combination on beatlactamase-resistant of acinetobacter baumannii isolated from patients admitted to the intensive care units to the shahid rajaii hosp
Saber Noshadi,
1 Alireza khodavandi,
2,* Fatemeh hosseini,
3
1. Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran
2. Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran
3. Department of Biolochsgy,Gachsaran Branch, Islamic Azad University,Gachsaran,Iran
Abstract
Introduction
: currently ,the most important factor in resistance to beta-lactam antibiotics in acinetobacter family is extended-spectrum beta-lactamases (esbl) the most common esbl enzymes. combination therapy would be one of the best strategies for the treatment of esbl-producing bacteria, due to increasing of drug resistance
Methods
In this study,50 isolatates of esbl- acinetobacter baumannii isolated from patients admitted to the intensive care units were identified via biochemical and bacteriological methods.then ,the antibacrerial activities of imipenem and sulbactam were evaluated in vitor alone and in combination using disk diffusion agar and broth microdilution tests according to clsi(m100 – s25 )for bacteria acinetobacter baumannii atcc29212 as a reference quality-control strains was employed.
Results
The mics range of imipenem and sulbactam against esbl were 0.125-512 respectively, while for imipenem in combination whit sulbactam was ranged from 0.128 to 8 ml .indeed , the combination therapy of imipenem/sulbactam was exerted the synergistic effects in esbls producing isoiates with fractional inhibitory concentration index ranged from 0.5 to 1.5 .
Conclusion
Our present study demonstrates that the combination of sulbactam with imipenem in incruases the efficacy of antibiotics , although the development of isolates of mdr and productive esls needs to be evaluated with other commonly used drug compounds.
Keywords
Acinetobacter, antibiotic resistance, extended-spectrum beta-lactamas
