1. Department of Biology East Azarbaijan Science and Research Branch, Islamic Azad University
: endometriosis is one of the most common causes of infertility in women. the cyp1a1 gene is one of the genes associated with this disease. air pollution is one of the factors that related with the expression of this gene. cyp1a1 is an enzyme produced by exposure to a stimulant, including smoke, when nicotine is ingested by blood in some tissues.
During the coordination with the staff member of the operating room of tabriz al-zahra educational hospital, before the surgery and after obtaining written consent from the patients, questions about the questionnaire were asked from the patients and recorded in the relevant forms. in this study, 30 samples of endometriosis tissue of the women exposed to cigarette smoke with their blood samples as the patient group, as well as 20 samples of women with normal endometrial tissue with their blood samples as the control group were collected. the tissue rna was extracted and cdna synthesized. the amount of gene expression was measured by real-time pcr. then, the rate of infection of women with nicotine (cotinine) was measured and compared by elisa method. finally, analysis of the results was done by spss software.
The expression level of cyp1a1 in the patient samples showed a significant increase compared to the control group, and the p-value was less than 0.003. there is also a significant relationship between the expression of cyp1a1 and the exposure to smoking (p-value = 0.0001). while there is no significant relationship between the amount of cotinine serum in the studied women and their exposure to smoking and not exposure to smoking (p-value = 0.618).
Increasing the expression of cyp1a1 gene in the tissues of the studied women was consistent with most studies in this field. in this study, women with endometriosis exposed to smoking seem to have high expression in the cyp1a1 gene, as opposed to women who are not exposed to smoking. considering the fact that there is no significant relationship between serum levels and cotinine protein levels in the two groups, and since, besides nicotine, there are many factors that have been identified in cigarette smoke that can apply their pathogenic effects to humans, it is included that other materials other than nicotine in cigarettes affect this process of increasing gene expression. cigarette smoke as an environmental risk factor can lead to increased expression of the cyp1a1 gene and an effect on endometriosis.