A comparison study to investigate the effect of condition medium derived from human adipose and cord blood mesenchymal stem cells on versican gene expression in normoxic and hypoxic peripheral bold mo
1. Tehran University of Science Sciences 2. Tehran University of Science Sciences
Abstract
Introduction
Mesenchymal stem cells (mscs), as one of the most important candidates in cell therapy, are suggested for treating a variety of diseases. recent studies have shown that these cells, after transplantation, can help repair damaged tissue through paracrine effects. one of the important factors in repairing damaged tissues is versican protein. the use of supernatant of cell culture derived from the cultivation of mscs with peripheral blood mononuclear cells in hypoxia conditions, assuming an increase in the versican factor, could be a good alternative instead of transplantation of mesenchymal stem cells
Methods
Mesenchymal stem cells derived from fat tissue and wharton jelly were evaluated using a flow cytometry and cd105, cd73 and cd90 surface markers. then, peripheral blood mononuclear cells were isolated and these cells were cultured with a soup derived from mesenchymal stem cells. to create hypoxia conditions, both deferroxamine and cobalt chloride were added to peripheral blood mononuclear cells media. then expression of versican gene in mononuclear cells was evaluated using the real time-pcr method.
Results
To confirm the hypoxia condition in peripheral blood mononuclear cells, western blotting was used to evaluate the hif-1α protein. flow cytometric results confirmed mesenchymal stem cells. on the other hand, the results of rt-pcr show that the expression of versican and vegf genes in hypoxia condition was significantly higher than that in normoxia condition in monouclear cells. western blotting also confirms hypoxia conditions in peripheral blood mononuclear cells. overall, the results show that factors secreted from mesenchymal stem cell increase the expression of the versican gene in mononuclear cells in hypoxia conditions as compared to normoxia.
Conclusion
Given the importance and applicability of mscs in cell therapy, it is suggested that the secreted factors of these cells can be replaced by direct injection of mscs in order to improve the tissue damage.
Keywords
Mesenchymal stem cells of adipose tissue and wharton jelly, mononuclear cells, hypoxia, versican gen
