The effects of bifidobacterium bifidum on infected wound healing in diabetic rats

Shahab Jamaran,1,*

1. Department of Microbiology, Science Faculty, Islamic Azad University of Arak, Iran



Probiotics are microorganisms in the digestive tract (gastrointestinal tract). in addition to helping the digestion of complex molecules, also it helps the immune system and reduces inflammation. the beneficial effects of this bacterium on gastrointestinal ulcer healing have been proven eventhough quantitative research has been done on the therapeutic effects of these bacteria on skin ulcers, especially the repair of skin lesions in diabetic people. the aim of this study was to investigate the effect of bifidobacterium bifidum on skin ulcer healing.


After isolating the probiotic bacteria strains and examining the production of exopolysaccharides, bifidobacterium bifidum with the ability of producing exopolysaccharides, were selected. then, wistar male rats were divided into 2 groups: control group and experimental group . a square scar in the size of 1.5 × 1.5 cm on mice back was made bifidobacterium bifidum probiotics that were added to eucerin. a control group was treated with eucerin without any probiotic clinical changes and histopathological effects of bifidobacterium bifidum probiotic on wound healing were evaluated every day and on 1, 3, 7, 14 and 21 days post-operation respectively


The results of the study were analyzed using the mean ± sd standard deviation and analyzed by anova. there was also a progressed increase in wound healing, but there was no significant difference between control and experimental groups. the difference is in the number of immune cells (neutrophils, macrophage and lymphocytes)


In this study, we observed that the probiotic bacteria consumed decreased inflammation and contributed to the process of wound healing in the experimental groups.


Bifidobacterium bifidum, probiotic, infected wound