Biological effects due to reduced expression of human hla-g4 and g5 isoforms in women with unexpected miscarriage

Neda Yaghoubi,1 Faramarz farzad,2,*

1. Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IRAN
2. Department of Immunology, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, IRAN



Human leukocyte antigen-g, an immune tolerance effector molecule, has important roles in the prevention of recurrent spontaneous abortions. to determine which isoforms are more probable to affect fetus loss, we evaluated the expression of seven hla-g4 and g5 isoforms and their effect in threatened-abortion women compared with controls.


A total of 101 women with threatened abortion in comparison to healthy controls, were included in this case-control study. all of the participants were aged between 20 to 32 years. quantitative real time pcr was used for evaluation of hla-g isoforms expression. soluble hla-g isoforms including hla-g2 and g4, were also measured through an immunoassay method.


Interestingly, our findings showed the reduced expression of hla-g4 and g5 isoforms. hla-g5 was the most effective isoform of hla-g in senile patients (p < 0.001). the most striking result to emerge from the data is that decreased expression of hla-g4 and g5 isoforms, may be the most probable cause of spontaneous abortion.


These results provide further support for the hypothesis that hla-g may have significant roles in pregnant women and can be considered as an important protective marker of unexpected abortion.


Hla-g; soluble hla g; threatened abortion; pregnant women