Carvacrol enhances skin flap survival by affecting on expression of cell apoptosis regulator pathways (bax, bcl2 ) and ros-involved proteins
Amir ali Ebadi fard azar,
1 Korosh khanaki,
2 Majid banimohammad,
3 Hamed shoorei,
4 Amirali ebadi fard azar,
5 Abbas majdi seghinsara,
6,*
1. Anesthesiologist and pain fellowship, Rasoul Akram Medical Center, Iran University of Medical Sciences, Tehran, Iran
2. Medical Biotechnology Research Center, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran
3. Physiology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
4. Department of Anatomical Science, Faculty of Medicine, Tabriz University of Medical Science, Tabriz, Iran.
5. Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
6. Department of Anatomical Science, Faculty of Medicine, Tabriz University of Medical Science, Tabriz, Iran.
Abstract
Introduction
Application of skin flaps is a main strategy for treatment of skin
lesions but proper treatments should be applied to improve skin flap survival rate and achieve higher and better treatment quality. if we could
decrease mentioned events and increase expression of the cell-survival pathways, we would have more survival and more beneficial results.
Methods
Forty sprauge-dawley rats (180-240g) were divided into four
groups: (1)sham- skin was cut in 8×3 cm without elevation, (2)flap- two parallel incisions were
connected caudally and skin elevation, (3)low dose carvacrol+flap, and (4)high dose carvacrol+flap
Results
Carvacrol increased the viability of skin flaps, induced more neovascularization
specially in distal flap areas, decreased mda contents, increased sod and decreased mpo activities, decreased bax and increased bcl-2 expression.
Conclusion
Our results proved that by administrating a therapeutic agent
(carvacrol), samples show decrease in markers of ir-derived ros injury like these studies.
Keywords
Carvacrol, skin flap, bax, bcl2, apoptosis, oxidative stress.
