Curcumin-entrapped muc-1 aptamer targeted dendrimer-gold hybrid nanostructure as a theranostic system for colon adenocarcinoma

Mahsa Shahriari,1,* Mona alibolandi,2 Mohammad ramezani,3 Khalil abnous,4

1. Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences,
2. Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences,
3. Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences,
4. Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences,

Abstract

Introduction

Gold nps have great potential in biomedical applications. pamam dendrimers are spherical, hyper branched macromolecules which can encapsulate therapeutic molecules while stabilizing metal nanoparticle such as gold nps. the aim of the current study was to investigate the theranostic capability of curcumin-loaded dendrimer gold hybrid structure.

Methods

Dendrimer-gold hybrid structure was synthesized by complexing aucl4− ions with pegylated amine-terminated generation 5 poly (amidoamine) dendrimer. the resultant hybrid system was loaded with curcumin. the curcumin-loaded pegylated au dendrimer was further conjugated to muc-1 aptamer in order to target the colorectal adenocarcinoma in vitro and in vivo.

Results

Obtained results demonstrated that the targeted theranostic agent was accumulated in ht29 and c26 cells in vitro and showed higher cellular cytotoxicity in comparison with non-targeted system. on the other hand, in vivo experiment demonstrated the potential of targeted theranostic system in ct-scan tumor imaging as well as cancer therapy.

Conclusion

Findings from this study suggested that muc-1 targeted curcumin-loaded pegylated au dendrimers have good x-ray attenuation and is desirable probe for ct imaging while demonstrating high therapeutic index against colorectal cancer adenocarcinoma.

Keywords

Pamam, curcumin, colorectal cancer, theranostic, ct scan