Investigating the effect of pegylation of nano-liposomes containing mentha piperita essential oil on the pattern of essential oil release in temperature and ph of cancerous cells

Mohammad Majdizadeh,1,* Bibi fatemeh haghiralsadat,2 Elyas sharifi,3

1. MSc. Cellular and Molecular Biology, Nano-Biotech Foresight Company Biotechnology Campus, Science & Technology Park of Yazd, Yazd, Iran
2. Department of Advanced Medical Sciences and Technologies, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3. MSc. Biophysics, Department of Biology, Taft PNU, Yazd, Iran

Abstract

Introduction

Herbal compounds have fewer side effects than chemical drugs but conventional prescribing of them faces serious challenges such as; digestive system low absorption, oxidizing some of the essential oil active ingredients along the way, and undesirable effect on non-target organs, etc. therefore identifying new strategies for the purpose of improvement of the drug delivery system of medicinal herbs is essential. one of the common pharmaceutical strategies to overcome these challenges is to use liposomal nano-carriers. in this study, a slow-release liposomal system containing peppermint essential oil was prepared and the effect of polyethylene glycol on the release of essential oil in cancerous cell conditions was investigated.

Methods

Liposomal vesicles were prepared using phosphatidylcholine, cholesterol and peg by thin-film method. mentha piperita’s essential oil were loaded into the liposomes using inactive loading method. then using the zeta sizer device the size and potential of zeta nano-carriers were measured and at the end essential oil release amount was calculated in healthy and cancerous cells condition ( 37° c, ph=7.4 and 42° c, ph=5.4)

Results

Liposomal vesicles containing mentha piperita’s essential oil showed the size of 221nm, 63.22% essential oil encapsulation efficiency, -45.77mv of zeta potential. the maximum release rate of the drug for this nano-carrier pegylated in healthy and cancerous cells condition was 41.46% and 45.16% respectively and the maximum release rate of the drug for this nano-carrier non pegylated in healthy and cancerous cells condition was 44.35% and 51.13% respectively.

Conclusion

In the present study, mentha piperita’s essential oil encapsulated in liposomal carriers. the result of this study showed that liposomal system containing mentha’s essential oil is slow-released in healthy and cancerous cells condition. also specified the use of polyethylene glycol in the manufacture of nano-liposomes containing essential oil of mentha piperita causes the release of essential oil from nano-liposomes to be reduced. the analysis of the drug release profile shows that in healthy and cancerous conditions, the essential oil release pattern has two phases; in the first phase, its release rate is high and after a while it will decrease. this difference seems to be due to the difference in the concentration of essential oil between the carrier and the buffer at the beginning and end of the essential oil release. in 2018, baba safari et al., produced nano-liposomes containing thyme essential oil with a size of 123 nm, zeta potential of 12 and an percent encapsulation of 23. their research results are close to the present study. in 2018, nadrinezhad et al., produced nano-liposomes containing thyme essential oil with a size of under 100 nm, zeta potential of between -26 and -36 mv and an percent encapsulation of 80%. their research results are close to the present study. in 2018, baba safari et al., produced nano-liposomes containing thyme essential oil with a size of under 100 nm, zeta potential of between -26 and -36 mv and an percent encapsulation of 80%.. in 2018, mazloom et al., produced nano-niosome containing mentha piperita essential oil with a size of under 80 nm, zeta potential -46.11 mv and an percent encapsulation of 61.36%. in 2018, majdizadeh et al., produced nano-liposomes containing mentha piperita essential oil with a size of 247 nm, zeta potential -34.54 mv and an percent encapsulation of 61.38%. in this study, maximum release of essential oil was reported at 32.23% for 60 hours.the results of the reported studies are very close to the present study. therefore, it can be concluded that pegilated nano-liposomes can be a suitable carrier to essential oil mentha piperita.

Keywords

Liposome, essential oil liberation, mentha piperita